Publications

A Versatile, Simple, and Rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications

Authors: Kavitha MS, Gayatri Kandpal, Jyothilakshmi S, V.R. Akshay Das, Nirmegh Basu, Athulya PS, V. Stalin Raj*

Journal: Methods

Year: 2026

DOI: https://doi.org/10.1016/j.ymeth.2026.04.005

NEW!

2026

Lumazine Synthase Nanoparticles as a Versatile Platform for Multivalent Antigen Presentation and Cross-Protective Coronavirus Vaccines

Authors: Jeswin Joseph, Kavitha MS, Priyanca Ahlawat, Malavika Prakash, Gayatri Kandpal, Gowtham Raj, Harshal Srivastava, P Charulekha, Aswin K Dev, Akshaya Radhakrishnan, Virendra Singh, Rahul Yadav, Purnima Chandramohanan, Reji Varghese, Zaigham Abbas Rizvi, Amit Awasthi, and V. Stalin Raj*

Journal: ACS Nano

Year: 2025

DOI: https://doi.org/10.1021/acsnano.5c06081

2025

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Internalization Does Not Rely on DPP4 Cytoplasmic Tail Signaling

Authors: Karthika T, Shubham Divakar, Gayatri Kandpal, Ann Mary Isaac, Kavitha MS, and V. Stalin Raj*

Journal: npj Viruses

Year: 2025

DOI: https://doi.org/10.1038/s44298-024-00080-y

2025

SARS‐CoV‐2 neutralizing antibody epitopes are overlapping and highly mutated which raises the chances of escape variants and requires the development of broadly reactive vaccines

Authors: Jeswin Joseph, Sukhada Darpe, Grishma Kulkarni, and V. Stalin Raj*

Journal: Proteins Structure, Function, and Bioinformatics

Year: 2023

DOI: https://doi.org/10.1002/prot.26488

2023

The use of pseudotyped coronaviruses for the screening of entry inhibitors: Green tea extract inhibits the entry of SARS-CoV-1, MERS-CoV, and SARS-CoV-2 by blocking receptor-spike interaction

Authors: Jeswin Joseph, Karthika T., V. R. Akshay Das, and V. Stalin Raj*

Journal: Current Pharmaceutical Biotechnology

Year: 2022

DOI: 10.2174/1389201022666210810111716

2022

SARS-CoV-2 Cellular Entry Is Independent of the ACE2 Cytoplasmic Domain Signaling

Authors: Karthika, T., Jeswin Joseph, V.R. Akshay Das, Niranjana Nair, Charulekha P., Melvin D. Roji, and V Stalin Raj*

Journal: Cells

Year: 2021

DOI: https://doi.org/10.3390/cells10071814

2021

Epigallocatechin-3-gallate (EGCG): a potential molecule for the development of therapeutics against emerging SARS-CoV-1, MERS-CoV and SARS-CoV-2 coronaviruses.

Authors: Jeswin Joseph, Karthika T., V.R. Akshay Das, & V. Stalin Raj*

Journal: Journal of Global Antimicrobial Resistance

Year: 2021

DOI: https://doi.org/10.1016/j.jgar.2021.05.005

2021

Design of a highly thermotolerant, immunogenic SARS-CoV-2 spike fragment.

Authors: Malladi SK, Singh R, Pandey S, Gayathri S, Kanjo K, Ahmed S, Khan MS, Kalita P, Girish N, Upadhyaya A, Reddy P, Pramanick I, Bhasin M, Mani S, Bhattacharyya S, Joseph J, Thankamani K, V. Stalin Raj, Dutta S, Singh R, Nadig G, Varadarajan R.

Journal: Journal of Biological Chemistry

Year: 2020

DOI: 10.1074/jbc.RA120.016284

2020

Selected Articles (Prior Work)

Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection. V. Stalin Raj *, Okba NMA, Gutierrez-Alvarez J, Drabek D, van Dieren B, Widagdo W, Lamers MM, Widjaja I, Fernandez-Delgado R, Sola I, Bensaid A, Koopmans MP, Segalés J, Osterhaus ADME, Bosch BJ, Enjuanes L, Haagmans BL*. Science Advances, 2018.
Identification of HCV Resistant Variants against Direct-Acting Antivirals in Plasma and Liver of Treatment-Naïve Patients. V. Stalin Raj, Hundie GB, Schürch AC, Smits SL, Pas SD, Le Pogam S, Janssen HLA, de Knegt RJ, Osterhaus ADME, Najera I, Boucher CA, Haagmans BL. Scientific Report, 2017, doi: 10.1038/s41598-017-04931-y.
Miscarriage Associated with Zika Virus Infection. Annemiek A. van der Eijk, Perry J. van Genderen, Rob M. Verdijk, Chantal B. Reusken, Ramona Mögling, Jeroen J.A. van Kampen, W. Widagdo, Georgina I. Aron, Corine H. GeurtsvanKessel, Suzan D. Pas, V. Stalin Raj, Bart L. Haagmans, Marion P.G. Koopmans. New England Journal of Medicine, 2016, doi: 10.1056/NEJMc1605898.
Naturally Occurring Recombination in Ferret Coronaviruses Revealed by Complete Genome Characterization. Mart M. Lamers, Saskia L. Smits, Gadissa B. Hundie, Marion Koopmans, Albert D.M.E. Osterhaus, Bart L. Haagmans*, V. Stalin Raj*. Journal of General Virology, 2016. doi: 10.1099/jgv.0.000520.
Deletion Variants of Middle East Respiratory Syndrome Coronavirus from Humans, Jordan, 2015. Mart M. Lamers*, V. Stalin Raj*, Mah’d Shafei*, Sami Sheikh Ali, Sultan M. bdallh, · Mahmoud Gazo, · Samer Nofal, · Xiaoyan Lu, Dean D. Erdman, Marion P. Koopmans, Mohammad Abdallat, Aktham Haddadin, Bart L. Haagmans. Emerging Infectious Diseases, 2016. doi: 10.3201/eid2204.152065.
Identification of protein receptors for coronaviruses by mass spectrometry. V. Stalin Raj., M. M. Lamers, S. L. Smits, J. A. Demmers, H. Mou, B. J. Bosch, and B. L. Haagmans. Methods in Molecular Biology 2015. doi: 10.1007/978-1-4939-2438-7_15.
Isolation of MERS Coronavirus from Dromedary Camel, Qatar, 2014. V. Stalin Raj., E. A. B. A. Farag, C. B. E. M. Reusken, M. M. Lamers, S. D. Pas, J. Voermans, S. L. Smits, A. D. M. E. Osterhaus, N. Al-Mawlawi, H. E. Al-Romaihi, M. M. AlHajri, A. M. El-Sayed, K. A. Mohran, H. Ghobashy, F. Alhajri, M. Al-Thani, S. A. Al-Marri, M. M. El-Maghraby, M. P. G. Koopmans, and B. L. Haagmans. 2014.Emerging Infectious Diseases, 2014. doi: 10.3201/eid2008.140663.
Adenosine Deaminase Acts as a Natural Antagonist for Dipeptidyl Peptidase 4-Mediated Entry of the Middle East Respiratory Syndrome Coronavirus. V. Stalin Raj., S. L. Smits, L. B. Provacia, J. M. A. van den Brand, L. Wiersma, W. J. D. Ouwendijk, T. M. Bestebroer, M. I. Spronken, G. van Amerongen, P. J. M. Rottier, R. A. M. Fouchier, B. J. Bosch, A. D. M. E. Osterhaus, and B. L. Haagmans. Journal of Virology, 2014, doi: 10.1128/JVI.02935-13.
Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation. Haagmans, B. L*., S. H. S. Al Dhahiry*, C. B. E. M. Reusken*, V. Stalin Raj*, M. Galiano, R. Myers, G. J. Godeke, M. Jonges, E. Farag, A. Diab, H. Ghobashy, F. Alhajri, M. Al-Thani, S. A. Al-Marri, H. E. Al Romaihi, A. Al Khal, A. Bermingham, A. D. M. E. Osterhaus, M. M. AlHajri, and M. P. G. Koopmans. The Lancet Infectious Diseases, 2014. doi:10.1016/S1473-3099(13)70690-X.
Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC. V. Stalin Raj., H. H. Mou, S. L. Smits, D. H. W. Dekkers, M. A. Muller, R. Dijkman, D. Muth, J. A. A. Demmers, A. Zaki, R. A. M. Fouchier, V. Thiel, C. Drosten, P. J. M. Rottier, A. D. M. E. Osterhaus, B. J. Bosch, and B. L. Haagmans. Nature, 2013. doi: 10.1038/nature12005.
MERS: emergence of a novel human coronavirus. V. Stalin Raj, A. D. M. E. Osterhaus, R. A. M. Fouchier, and B. L. Haagmans. Current Opinion in Virology 2014.
Novel Hepatitis E Virus in Ferrets, the Netherlands. V. Stalin Raj, S. L. Smits, S. D. Pas, L. B. V. Provacia, H. Moorman-Roest, A. D. M. E. Osterhaus, and B. L. Haagmans. Emerging Infectious Diseases, 2012, doi: 10.3201/eid1808.111659.
Effect of temperature and salinity on the infectivity pattern of white spot syndrome virus (WSSV) in giant tiger shrimp Penaeus monodon. Stalin Raj., K. K. Vijayan, S. V. Alavandi, C. P. Balasubramanian, and T. C. Santiago. (Fabricius, 1837). Indian Journal of Fisheries, 2012.
Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells. V. Stalin Raj, G. Fournier, K. Rakus, M. Ronsmans, P. Ouyang, B. Michel, C. Delforges, B. Costes, F. Farnir, B. Leroy, R. Wattiez, C. Melard, J. Mast, F. Lieffrig, and A. Vanderplasschen. Veterinary Research, 2011. (with cover page illustration)
The Major Portal of Entry of Koi Herpesvirus in Cyprinus carpio Is the Skin. Costes, B*., V. Stalin Raj*, B. Michel, G. Fournier, M. Thirion, L. Gillet, J. Mast, F. Lieffrig, M. Bremont, and A. Vanderplasschen. Journal of Virology, 2009. (with cover page illustration.
Virulence status, viral accommodation and structural protein profiles of white spot syndrome virus isolates in farmed Penaeus monodon from the southeast coast of India. Stalinraj, V., K. K. Vijayan, M. Sanjuktha, C. P. Balasubramanian, S. V. Alavandi, and T. C. Santiago. Aquaculture Research, 2009.
Cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in Cyprinus carpio koi. Costes, B., G. Fournier, B. Michel, C. Delforge, V. Stalin Raj, B. Dewals, L. Gillet, P. Drion, A. Body, F. Schynts, F. Lieffrig, and A. Vanderplasschen. Journal of Virology, 2008 (with cover page illustration)
Incidence of white muscle disease, a viral like disease associated with mortalities in hatchery-reared postlarvae of the giant freshwater prawn Macrobrachium rosenbergii (De Man) from the south-east coast of India.Vijayan, K. K., V. Stalin Raj, S. V. Alavandi, V. T. Sekhar, and T. C. Santiago. Aquaculture Research, 2005.
Polychaete worms - a vector for white spot syndrome virus (WSSV). Vijayan, K. K., V. Stalin Raj, C. P. Balasubramanian, S. V. Alavandi, V. T. Sekhar, and T. C. Santiago. Diseases of Aquatic Organisms, 2005.
White Muscle Syndrome- afflicted Macrobrachium rosenbergii need for health management. K.K. Vijayan, V. Stalin Raj, S.V. Alavandi, V. Thillai Sekhar, B. Vaseeharan, T.C. Santiago. Fishing Chimes, 2003.